Genetic properties of bla CTX-M, bla SHV and bla TEM genes in ESBL producing E. coli clinical isolates

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Multiplex PCR for bla(CTX-M) & bla(SHV) in the extended spectrum beta lactamase (ESBL) producing gram-negative isolates.

BACKGROUND & OBJECTIVE Phenotyping is commonly used for detection of extended spectrum beta lactamase (ESBL) production in gram-negative isolates. ESBLs are mainly coded for by three important genes, namely bla(TEM), bla(SHV) and bla(CTX-M). In this study we used a multiplex PCR as a rapid method to identify two common genes (bla(CTX-M) & bla(SHV)) responsible for extended spectrum beta lactama...

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High prevalence of bla(CTX-M) extended spectrum beta-lactamase genes in Klebsiella pneumoniae isolates from a tertiary care hospital: first report of bla(SHV-12), bla(SHV-31), bla(SHV-38), and bla(CTX-M-15) in Brazil.

The aim of this study was to investigate the presence and prevalence of bla(TEM), bla(SHV), and bla(CTX-M) and bla(GES)-like genes, responsible for extended spectrum beta-lactamases (ESBLs) production in clinical isolates of Klebsiella pneumoniae collected from a Brazilian tertiary care hospital. Sixty-five ESBL producing K. pneumoniae isolates, collected between 2005 and 2007, were screened by...

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frequency of bla tem, bla shv, bla ctx-m, and qnra among escherichia coli isolated from urinary tract infection

conclusions considering the high prevalence of esbl genes (bla ctx-m, 87%; and bla tem, 82%), fluoroquinolones may be used as an alternative drug in treatment, although resistance to this family is increasing as well. as a result, this increasing trend should be prevented using appropriate guidelines for prescription. background escherichia coli is the most important as well as the most common ...

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detection of bla tem and bla shvgenes among clinical isolates of e. coli from tehran hospitals

background and objectives: the pathogenic strains of escherichia coli cause severe septicemia, urinary tract infection and wound sepsis. resistance of this organism to the expanded-spectrum cephalosporins occurs via the production of extended-spectrum β-lactamases (esbls) such as tem and shv. these enzymes hydrolyze oxyimino cephalosporins (cefotaxime, ceftazidime) and monobactams (aztreonam). ...

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Spread of bla(CTX-M-type) and bla(PER-2) beta-lactamase genes in clinical isolates from Bolivian hospitals.

OBJECTIVES To assess the prevalence and types of genes encoding extended-spectrum beta-lactamases (ESBLs) in clinical isolates of Enterobacteriaceae, Pseudomonas aeruginosa and Acinetobacter spp. from Bolivia. METHODS A total of 642 clinical isolates were collected consecutively during a 4 month period (September to December 2004). Resistance or reduced susceptibility to cefotaxime and/or cef...

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ژورنال

عنوان ژورنال: Journal of Medical Care Research and Review

سال: 2021

ISSN: 2589-8949,2589-8930

DOI: 10.15520/mcrr.v4i1.157